Gene description for CYB5R3
Gene name cytochrome b5 reductase 3
Gene symbol CYB5R3
Other names/aliases B5R
DIA1
Species Homo sapiens
 Experiment description of studies that identified CYB5R3 in Embryonic kidney cells

1
Experiment ID1659
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

2
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

3
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

5
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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